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To further investigate the molecular processes involved in the transition from pluripotency to bipotency, we examined the transcriptome changes in the mac1 mutant more thoroughly at both the 0.2 mm and 0.4 mm stages in direct comparison to… To isolate OMVs, 20 ml of supernatant was subjected to ultracentrifugation (Thermo Scientific S50-A rotor; Thermo Fisher Scientific, Waltham, MA) at 209,438 × g for 1.5 h at 4°C. Pelleted vesicles were resuspended in 500 μl of phosphate…

The ALX sports top-of-the-line processing power (a 3.6-GHz Pentium or a 2.4-GHz AMD, as of this writing) and up to 4 gigs of RAM.

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